Professor David Haylock

 - Affiliate Investigator

Research focus: Development of bioreactor technology for blood cell growth 

Professor David Haylock brings considerable expertise in the isolation, characterization and ex vivo manipulation of human haemopoietic stem and progenitor cells to this initiative. Specifically, Professor Haylock has developed serum-free cytokine dependent culture systems for ex vivo expansion of haemopoietic stem cells (HSC) that enable analysis of the effect of purified recombinant cytokines on the proliferation and differentiation of HSC. Furthermore, he has significant skills in flow cytometric methods for multiparameter immunophenotypic analysis and fluorescent activated sorting of rare cell subsets.

Recently, in collaboration with Professors Elefanty and Stanley (Monash University), this has involved detailed immunophenotypic characterisation of putative haemopoietic stem cells derived from differentiated hESC cultures. In addition, through his direction of the CRC-Polymers biomaterials project Prof Haylock has developed knowledge and expertise on biomaterials and bioreactor based systems for expansion of haemopoietic stem and progenitor cells. 

This includes the development of modified polymer surfaces that facilitate immobilisation of biological ligands for cell expansion. These skills and expertise will be utilised in development of novel systems for propagating megakaryocytes and producing platelets from hESC derived precursors.