Beckman Coulter CytoFLEX S

The Beckman Coulter CytoFLEX S analyser has 4 lasers and 13 fluorescent detectors + FSC, SSC. This instrument also has a violet SSC detector for nano-particle analysis. Software: CytExpert

Laser/ Filter combination 

Fluorochromes

Violet (405nm):  450/45

DAPI,  BV421, Pacific Blue, V450, AF405, Cascade Blue, Dyecycle violet

Violet (405nm): 525/40

V500, AmCyan, BV510, Live/dead aqua, CFP

Violet (405nm): 610/20

BV605

Violet (405nm): 660/20

BV650

Blue (488nm): 525/40

FITC, AF488, GFP, CFSE, YFP, Dyecycle green, FAM

Blue (488nm): 690/50

7AAD, PI, PerCP-Cy5.5, PerCP

Y/G (561nm): 585/42

PE, mOrange, AF568, dTomato, TAMRA

Y/G (561nm): 610/20

PE-Texas Red, PE-AF610, RFP, DsRed, mCherry, AF594, Texas Red

Y/G (561nm): 690/50

PE-Cy5, 7AAD, PI

Y/G (561nm): 780/60

PE-Cy7, PE-AF750, PE-vio770

Red (633nm): 660/20

APC, AF633, AF647, APC-Cy5, Sytox Red dead

Red (633nm): 712/25

AF700

Red (633nm): 780/60

APC-Cy7, APC-AF750, APC-H7

 

Additional filter sets:

780/60

510/20

710/50

585/15

V405/10 (SSC for nano-particle detection)