"Tracking Single Cell Fate in Haematopoiesis" to be presented by Shalin Naik PhD, Laboratory Head, Molecular Medicine Division, Walter & Eliza Hall Institute
TIME: 04:00 PM – Tuesday, 4 March, 2014 (Refreshments follow on Level 5 at 5.00pm)
VENUE: Level 5 Seminar Room, Melbourne Brain Centre, Parkville Campus
ABSTRACT: A true sports fanatic would never be satisfied with a simple win, lose or draw result. It offers nothing of the complexity of the game; how the score fluctuated, which players scored, who was injured, who was the MVP? In that same way, Developmental biologists should be interested in the end result of a population of stem and progenitors, but rather how every single one of those contributes to their tissue of interest. To track the fate of single haematopoeitic progenitors in vivo, we used a technology termed cellular barcoding. Here, we use lentivirus to deliver unique 100 nt DNA barcodes into the genome of cells, such that daughter cells inherit that barcode. At a later time, we utilise next-gen sequencing technology to profile the barcode signatures in each progeny cell type, thereby establishing the presence of mono-, oligo- and multi-outcome stem and progenitor fate. We reveal that, unlike the paradigm that states that every MPP or HSC generates every lineage, there are various patterns of lineage output already prevalent at this stage including bias for myeloid, lymphoid but also dendritic cell lineages. We also propose a revised ‘graded commitment’ model of haematopoiesis to explain the gradual accumulation of fate in development rather than an ‘all-or-none’ bifurcation at a particular point.
BIO: Dr Naik is a graduate of the University of QLD (Microbiology & Biochemistry) where he did his Honours with Prof. David Hume on macrophage activation by CpG DNA. After a 2 year hiatus in London where he worked as a waiter, graphics and production manager for Citibank, and TV presenter for a show about extreme weather phenomenon, he returned to Melbourne to do his PhD with Prof. Ken Shortman on dendritic cell development at WEHI. It was here he gained an interest in single cell tracking and fate determination in biology, and was awarded his PhD in 2006. Interested in the emerging technology of ‘cellular barcoding’ Dr Naik did his postdoc in the laboratory of Prof. Ton Schumacher at the Netherlands Cancer Institute, where he traced the single cell output of haematopoietic stem and progenitor cells in vivo. After returning to WEHI in 2013, he was later appointed as a Laboratory Head in the Molecular Medicine Division where he studies single cell fate using different technologies.