Join us to hear A/Prof Fredrick Lanner from the Karolinska University Hospital discuss how creating a map of transcriptional activity during embryo development reveals much about lineage formation and X-chromosome activation.
DATE: Monday 15 February 2016
TIME: 12 noon
VENUE: Level 5, Seminar Room, Melbourne Brain Centre, The University of Melbourne
TITLE:Single-cell RNA-seq reveal lineage formation and X-chromosome dosage compensation in human preimplantation embryos
ABSTRACT: Mouse studies have been instrumental in forming our current understanding of early cell-lineage decisions in mammals, however similar insights into early human development are severely limited. To generate a comprehensive transcriptional map of human embryo formation we sequenced the transcriptomes of 1,469 individual cells from 79 human embryos during preimplantation development. Analyses of these data revealed when and how the first cell-lineages differentiate, and that the segregation of the trophectoderm, epiblast and primitive endoderm lineages are established in concert and coincide with blastocyst formation. We also provide a new model for female X-chromosome dosage compensation in the human preimplantation embryo.
BIO:Fredrik Lanner received his PhD at Karolinska institute (KI) studying vascular development (2008). In 2009 he moved to Toronto and did postdoctoral research in Janet Rossants lab at the Hospital for Sick Children with focus on mouse preimplantation development as well as regulation of pluripotent stem cells. In 2012, Fredrik Lanner moved back to KI and have now established an independent lab consisting of 3 PhD students and 4 postdocs at the Department of Clinical Science, Intervention and Technology.
The lab is focused on exploring the mechanisms that control the first week of mammalian preimplantation development from a single fertilized egg to a multicellular embryo with distinct tissues. Understanding this period of development is of great importance both for current assisted reproductive technologies and future regenerative medicine using embryonic stem cells.
Our current knowledge about preimplantation biology has almost exclusively been worked out using the mouse model. Early human development on the other hand, which clearly is the organism that we need to understand the best, is strikingly understudied. Our research is therefor focused on extending our understanding of early mammalian development and translating knowledge gained in the mouse model to the human setting.
Recently we have also initiated a project directed towards stem cell based regenerative medicine to treat age-related macular degeneration. We are focusing on establishment of clinically compliant and GMP quality in vitro differentiation strategies to generate retinal pigmented epithelial cells and photoreceptors. Together with Professor Outi Hovatta we are now also working to establish a GMP production of clinically compliant hESCs to be used by us and other collaborators when moving into clinical studies.