In the last two years, a number of studies highlighted that 6-Methyladenine (m6A) modifications on mRNA are central to the control of mouse embryonic stem cell (mESC) pluripotency. The RNA methyltransferase-like 3 (METTL3) is a depositor of m6A unto mRNA of key pluripotency transcription factors like NANOG. To address the role of METTL3 and m6A dependent circuitry in pluripotency, we combine unique reporter cell lines with single cell level, quantitative live cell imaging approaches. We discuss METTL3 and m6A dependent mechanisms involved in pluripotent cell state transitions.
1) Introduced to pluripotent embryonic stem cell research by Prof. Martin Pera at the Australian Stem Cell Center, Melbourne. Honors and PhD supervised by Prof. Pera, completed in 2006.
2) Postdoctoral work initially at the Hubrecht Laboratory, Utrecht, Holland and then Helmholtz Research Center in Munich, Germany.
3) At the Helmholtz research center, focused on live cell imaging approaches and stem cell pluripotency control.
4) Currently Project Group Leader at Oslo University Hospital and the Norwegian Stem Cell Center, focusing on pluripotency regulation and RNA plasticity.